THE ROLE OF INTESTINAL MICROBIOTA IN PARKINSON’S DISEASE PATHOGENESIS (EXPERIMENTAL STUDY)
Abstract
Parkinson’s disease (PD) is an extrapyramidal chronopathological process with specific motor disorders and non motor disorders complex accompanied by the change of normal dynamics of daily range of the majority of physiological processes in the organism. A special attention is given to the interrelation between microbiota intestines brain: the way microflora, faecal transplantation material, enteral nervous system and the brain black substance are connected with each other. Objective of the current study is modelling of Parkinson’s disease in experimental animals to make a subsequent comparative analysis of various approaches to anti-Parkinson’s disease therapies. The study involved 40 mature rats of the line divided into 4 experimental groups: 1 - “Control” (n=10); 2 - “Rotenone” (n=10); 3 - “Rotenone+amantadine” (n=10); 4 - “Rotenone+microbiota” (n=10). To confirm motor disorders the rotenone rats were tested by the programs “Open field” and “Bars”. Amantadine was chosen as a medical therapy due to its availability and effectiveness. Moreover, transplantation of faecal microbiota to experimental animals has not a small value in the treatment of Parkinson’s disease. According to the results of “Open field” and “Bars” tests the examined animals presented significant increase of upright postures and number of sniffing actions, and these indicators characterize motor activity of the experimental animals that could confirm the improvement of motor symptomatology. According to the test “Bars” we also observed that the examined animals undergone microbiota transplantations were characterized by a longer keeping on poles to be compared with the rats with rotenone Parkinson’s disease. Thus, it is possible to make a conclusion that behaviour tests used by us can prove the improvement of nervous muscular coordination and motor activity in the rats undergone microbiota transplantations in comparison with the group with rotenone Parkinson’s disease.
