Laser Doppler flowmetry for studying tissue blood flow in a model of carrageenan-induced edema in mice
PATHOPHYSIOLOGY — MEDICINE
Abstract
Introduction.Carrageenan-induced edema is the most commonin vivomodel for evaluating the therapeutic efficacy of anti-inflammatory drugs. The pathogenesis of carrageenan-induced edema is based on acute exudative inflammation, in which microcirculation disorders play an important role. The study of the pathogenesis of microcirculation is of great interest, both diagnostic and prognostic, in assessing the functional status of the organism. One of the promising and successfully applied diagnostic methods for studying tissue blood flow is laser Doppler flowmetry, which makes it possible to assess changes in microcirculatory processes.The aimis to evaluate tissue blood flow in mice using the laser Doppler flowmetry method in comparison with plethysmometry parameters in the carrageenan-induced edema model to study the efficacy and pharmacodynamics of biomedical cellular products and pharmaceutical substances in preclinical practice.Materials and methods.40 mice (20 males and 20 females) of the BALB/c line aged 8–10 weeks were used in the study. During the experiment, carrageenan-induced edema was modeled, laser Doppler flowmetry and plethysmometry were performed in the area of the left hind limb of the experimental animal. According to the results of the conducted research, the following tasks were performed: 1) assessment of tissue perfusion in mice in the area of plantar aponeurosis in dynamics; 2) plethysmometry in dynamics (assessment of the severity of edema in animals by changes in the volume of the animal’s paw); 3) comparison of plethysmometry and laser Doppler flowmetry. It was found that the degree of blood flow restoration and the severity of edema correlate with each other (the correlation coefficient is 96%).Conclusions.The use of laser Doppler flowmetry in the carrageenan-induced edema model in mice makes it possible to assess the state of microcirculationin vivoand dynamically. The method is characterized by accuracy and high sensitivity, which makes it possible to determine changes in blood microcirculation in the early stages, including before the “visual” manifestation of edema. At the same time, there is a high correlation between the data obtained using laser Doppler flowmetry and plethysmometer. In the future, this method can be widely used in preclinical practice to evaluate the therapeutic efficacy and pharmacodynamics of drugs under development that affect regional blood circulation and microcirculation.
References
1. Калакутский Л.И., Манелис Э.С. Аппаратура и методы клинического мониторинга. Самара: СГАУ; 1999. 360 с.
2. Методы ветеринарной клинической лабораторной диагностики. Кондрахин И.П., ред. М.: Колос С; 2005. 520 с.
3. Конг Хонг Хань, Хазиахметова В.Н., Зиганшина Л.Е. Моделирование воспалительных отеков: взаимозаменяемы ли модели? Экспериментальная и клиническая фармакология. 2015;78(7):24–31. EDN: UBIDQB.
4. Кошкин В.М. и др. Исследование микроциркуляции при хронической венозной недостаточности нижних конечностей. В.С. Савельев, ред. М.: Изд-во ГОУ ВПО РГМУ Росздрава; 2006. 25 с.
5. Webster J.G. Medical instrumentation. Application and design. John Wiley &Sons; 2009. 675 p.
6. Фетодов А.А., Акулов С.А. Измерительные преобразователи биомедицинских сигналов систем клинического мониторинга. М.: Радио и связь; 2013. 250 с.
7. Чуян Е.Н., Ананченко М.Н. Индивидуально-типологический подход к исследованию процессов микроциркуляции крови. Ученые записки Таврического национального университета им. В.И. Вернадского. Серия: Биология. Химия. 2009;22(3):159–173. EDN: VKWMXB.
8. Committee for the Update of the Guide for the Care and Use of Laboratory Animals. 8th Edition. National Research Council; 2010. 248 p.
9. Whiteley P.E., Dalrymple S.A. Models of inflammation: carrageenan-induced paw edema in the rat. Curr Protoc Pharmacol. 1998;5.4.1.-5.4.3. URL: https://www.sci-hub.ru/10.1002/0471141755.ph0504s00 (accessed: 06.01.2026).
10. Lin Q., Wu J., Willis W.D. Dorsal Root reflexes and cutaneous neurogenic inflammation after intradermal injection of capsaicin in Rats. Neurogenic Inflammation Mediated by DRRS. 1999;85(2):2602–2611.
11. Marris C.J. Carrageenan-induced paw edema in the rat and mouse. Methods Mol Biol. 2003;225:115–121. https://doi.org/10.1385/1-59259-374-7:115.
12. Шишкин А.Н., Николаева А.А. Особенности микроциркуляции при псориатическом артрите. Региональное кровообращение и микроциркуляция. 2021;20(3):11–17. https://doi.org/10.24884/1682-6655-2021-20-3-11-17. EDN: EDJZHJ.
13. Stefanovska A., Bracic M. Physics of the human cardiovascular system. Contemporary Physics. 1999;40(1);31–35.
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